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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which ends up in chromosome instability, and thus may lead to tumorigenesis. This protein was discovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important position in marketing cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but comprehensive nucleotide sequence information and facts is just not yet offered.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized with the antibody.
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This information outlines further more probable optimization of mobile and immunoassay parameters to guarantee the absolute best results are...
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This information outlines even further feasible optimization of mobile and immunoassay parameters to be sure the best possible results are...
Antibodies certain to the blocking peptide no longer bind towards the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates at the G1-S phase boundary which is degraded as cells progress through S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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